Transient Expression Here we use a transient expression system in the model plant Nicotiana benthamiana to reconstitute the two-step plant-derived biosynthetic pathway for auxin (indole acetic acid) to achieve accumulation up to 500 ng/g fresh mass (FM). Additionally, we have generated transgenic Nicotiana benthamiana lines that express fluorescent protein markers targeted to nuclei, endoplasmic reticulum or actin filaments. This high α-AIC3 accumulation allowed its structural and functional characterizations. Agroinfiltration in Nicotiana benthamiana leaves (Kapila et al., 1997; Yang et al., 2000), when combined with the coexpression of a suppressor of gene silencing (Silhavy et al., 2002; Voinnet et al., 2003), has established itself as the most utilized transient expression system in plants. (A) DAB staining of N. benthamiana leaves expressed with GFP, PvAvh74, PvAvh74+DPI, or PvAvh74+PvAvh8. Ghazala W, Varrelmann M: Tobacco rattle virus 29K movement protein is in Nicotiana benthamiana cell suspensions and hairy roots. ; Genre: Journal Article; Published online: 2020-11-20; Open Access; Title: Nicotiana benthamiana as a transient expression host to produce auxin analogs Improving agroinfiltration-based transient gene expression in ⦠Nicotiana benthamiana, which is now widely used for transient expression assays in plants, is an alternative host which may circumvent the need for optimization required with microbial hosts. Nicotiana benthamiana, a wild relative of tobacco, has become increasingly popular as a heterologous expression platform for reconstituting plant natural product pathways, because it is amenable to Agrobacterium-mediated transient expression, a scalable and highly flexible process that enables rapid expression of genes and enzymes from other plant species. Nicotiana benthamiana as a Transient Expression Host to ⦠Furthermore, the ectopic expression of LtCSEP1 in N. benthamiana enhanced disease susceptibility to L. theobromae by downregulating the defense-related genes. . qPCR represents the accumulation of NbSGT1 transcripts, normalized to NbEF1a, in NbSGT1 virus-induced gene silencing (VIGS) plants. RNA viral vectors for improved Agrobacterium-mediated transient ...
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